INSERM
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
Nihal E. Vrana 1, Agnes Dupret-Bories 1,2, Christophe Chaubaroux 1, Elisabeth Rieger 1,2, Christian Debry 1,2, Dominique Vautier 1,3, Marie-Helene Metz-Boutigue 1,3, Philippe Lavalle 1,3
1Biomatériaux et Bioingénieriee, INSERM, 2Service Oto-Rhino-Laryngologie, Hôpitaux Universitaires de Strasbourg, 3Faculté de Chirurgie Dentaire, Université de Strasbourg
In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.
Biochemistry
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Simon Leclerc 1,2, Youri Arntz 2, Yuichi Taniguchi 1,3
1Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, 2Laboratoire de Biomatériaux et Bioimagerie, INSERM, 3PRESTO, Japan Science and Technology Agency
Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.
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