Visualization of Endoplasmic Reticulum Subdomains in Cultured CellsMatteo Fossati 1,2,3, Nica Borgese 2,3,4, Sara Francesca Colombo 2,3, Maura Francolini 1,2
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine, University of Milan, 3Neuroscience Institute, National Research Council (CNR), 4Department of Health Science, "Magna Graecia" University of Catanzaro
We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.
