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Environmental Health Centre

2 ARTICLES PUBLISHED IN JoVE

Biology

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Jason M. O'Brien¹, Marc A. Beal¹, John D. Gingerich¹, Lynda Soper¹, George R. Douglas¹, Carole L. Yauk¹, Francesco Marchetti¹

¹Environmental Health Science and Research Bureau, Health Canada, Environmental Health Centre

De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.

Chemistry

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Nikolai L. Chepelev¹, Dean A. Kennedy¹, Remi Gagné¹, Taryn White¹, Alexandra S. Long¹, Carole L. Yauk¹, Paul A. White¹

¹Environmental Health Science and Research Bureau, Health Canada

The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.