Environmental Health Centre
2 ARTICLES PUBLISHED IN JoVE
Biology
Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
Jason M. O'Brien 1, Marc A. Beal 1, John D. Gingerich 1, Lynda Soper 1, George R. Douglas 1, Carole L. Yauk 1, Francesco Marchetti 1
1Environmental Health Science and Research Bureau, Health Canada, Environmental Health Centre
De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.
Chemistry
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Nikolai L. Chepelev 1, Dean A. Kennedy 1, Remi Gagné 1, Taryn White 1, Alexandra S. Long 1, Carole L. Yauk 1, Paul A. White 1
1Environmental Health Science and Research Bureau, Health Canada
The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.
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