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University Children's Hospital Münster

2 ARTICLES PUBLISHED IN JoVE

Medicine

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Markus Brückner^*1, Philipp Lenz^*1, Tobias M. Nowacki¹, Friederike Pott¹, Dirk Foell², Dominik Bettenworth¹

¹Department of Medicine B, University Hospital Münster, ²Department of Pediatric Rheumatology and Immunology, University Children's Hospital Münster

Small animal imaging techniques allow serial diagnostic examinations and therapeutic interventions in vivo. Recently, the scope of applications has significantly widened and currently includes assessment of colonic tumor development, wound healing and monitoring of inflammation. This protocol illustrates these diverse potential applications of murine endoscopy.

Immunology and Infection

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Sabrina Fuehner¹, Dirk Foell¹, Christoph Kessel¹

¹Department of Pediatric Rheumatology and Immunology, University Children's Hospital

This protocol describes a purification method for recombinant tag-free calcium binding protein S100A12 and its ion-induced oligomers for human monocyte stimulation assays.