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University of Firenze

1 ARTICLES PUBLISHED IN JoVE

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Biology

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Alessandra Stefan 1,2, Alessandro Ceccarelli 1, Emanuele Conte 1, Alejandro Montón Silva 1, Alejandro Hochkoeppler 1,2
1Department of Pharmacy and Biotechnology, University of Bologna, 2CSGI, Department of Chemistry, University of Firenze

Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.

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