JoVE Logo
Centre de ressources universitaires

S'identifier

University of Firenze

1 ARTICLES PUBLISHED IN JoVE

image

Biology

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Alessandra Stefan 1,2, Alessandro Ceccarelli 1, Emanuele Conte 1, Alejandro Montón Silva 1, Alejandro Hochkoeppler 1,2
1Department of Pharmacy and Biotechnology, University of Bologna, 2CSGI, Department of Chemistry, University of Firenze

Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.

JoVE Logo

Confidentialité

Conditions d'utilisation

Politiques

Recherche

Enseignement

À PROPOS DE JoVE

Copyright © 2024 MyJoVE Corporation. Tous droits réservés.