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King's College London BHF Centre

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Mei M. Wong^*1, Xuechong Hong^*1, Eirini Karamariti¹, Yanhua Hu¹, Qingbo Xu¹

¹Cardiovascular Division, King's College London BHF Centre

Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.