Production of Double-stranded DNA MinistringsShirley Wong 1, Peggy Lam 1, Nafiseh Nafissi 1, Steven Denniss 2, Roderick Slavcev 1
1School of Pharmacy, University of Waterloo, 2Sgd Health Innovation
The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.
