Purification of Mouse Brain VesselsAnne-Cécile Boulay 1,2,3, Bruno Saubaméa 4, Xavier Declèves 4, Martine Cohen-Salmon 1,2,3
1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot
We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.
