Ex vivo Live Imaging of Lung Metastasis and Their MicroenvironmentRenske J.E. van den Bijgaart *1, Niwen Kong *1, Carrie Maynard 1,2, Vicki Plaks 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.
