Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht

1 ARTICLES PUBLISHED IN JoVE

Medicine

Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

Renske J.E. van den Bijgaart^*1, Niwen Kong^*1, Carrie Maynard^1,2, Vicki Plaks^1,3

¹Department of Anatomy, University of California, ²Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine

We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.