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National Health Research Institutes, Taiwan

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Ching-Hui Lin^1,2, Hao-Chen Chang^1,2, Chia-Hsien Hsu^1,2,3

¹Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, ²Tissue Engineering and Regenerative Medicine, National Chung Hsing University, ³Institute of NanoEngineering and MicroSystems, National Tsing Hua University

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

Bioengineering

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Cheng-Kun He^1,2, Ya-Wen Chen³, Ssu-Han Wang³, Chia-Hsien Hsu^1,2

¹Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, ²Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, ³National Institute of Cancer Research, National Health Research Institutes

This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.