National Health Research Institutes, Taiwan
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Ching-Hui Lin 1,2, Hao-Chen Chang 1,2, Chia-Hsien Hsu 1,2,3
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University
Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.
Bioengineering
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
Cheng-Kun He 1,2, Ya-Wen Chen 3, Ssu-Han Wang 3, Chia-Hsien Hsu 1,2
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 2Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3National Institute of Cancer Research, National Health Research Institutes
This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.
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