Analysis of SCAP N-glycosylation and Trafficking in Human CellsChunming Cheng 1, Jeffrey Yunhua Guo 1, Feng Geng 1, Xiaoning Wu 1, Xiang Cheng 1, Qiyue Li 1, Deliang Guo 1
1Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine
We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP N-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.
