The Ohio State University Comprehensive Cancer Center and College of Medicine

1 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Chunming Cheng¹, Jeffrey Yunhua Guo¹, Feng Geng¹, Xiaoning Wu¹, Xiang Cheng¹, Qiyue Li¹, Deliang Guo¹

¹Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine

We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP N-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.