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The Research Institute at Nationwide Children’s Hospital

4 ARTICLES PUBLISHED IN JoVE

Medicine

Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System

Kelly Regan¹, Soheil Moosavinasab², Philip Payne¹, Simon Lin²

¹Department of Biomedical Informatics, The Ohio State University, ²Research Information Solutions and Innovation, The Research Institute at Nationwide Children’s Hospital

Here we describe a protocol using the web-based drug repurposing hypothesis generation tool: "RE:fine Drugs." This protocol can be modified to a user's preferences at the level of the query type (gene, drug or disease) and/or the range of available advanced options.

Genetics

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Camila F. Almeida¹, Emma C. Frair¹, Nianyuan Huang¹, Reid Neinast², Kim L. McBride^2,3,4,6, Robert B. Weiss⁵, Kevin M. Flanigan^1,6, Nicolas Wein^1,6

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, ³The Heart Center, Nationwide Children’s Hospital, ⁴Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, ⁵Department of Human Genetics, The University of Utah School of Medicine, ⁶Department of Pediatrics, The Ohio State University

This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.

Neuroscience

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Cassandra N. Dennys¹, Julieth A. Sierra-Delgado¹, Shrestha Sinha Ray¹, Annalisa M. Hartlaub¹, Florence S. Roussel¹, Yacidzohara Rodriguez¹, Kathrin Meyer^1,2

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²College of Medicine, The Ohio State University

We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

Medicine

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography

Qiang Zeng¹, Doug A. Gouchoe^2,3, Mahboubeh Nabavinia⁴, Yong Gyu Lee², Xi Wang², Terri A. Shaffer⁵, Mitchel R. Stacy^4,5,6, Blake R. Peterson^7,8, Bryan A. Whitson^2,9, Christopher Breuer^1,4, Sylvester M. Black^1,10

¹Department of Surgery, Nationwide Children’s Hospital, ²COPPER Laboratory, The Ohio State University, ³88th Surgical Operation Operations Squadron, WPAFB, ⁴Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, ⁵Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, ⁶Interdisciplinary Biophysics Graduate Program, The Ohio State University, ⁷Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, ⁸The Ohio State University Comprehensive Cancer Center, ⁹Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, ¹⁰Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.