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The University of Mississippi Medical Center

2 ARTICLES PUBLISHED IN JoVE

Biology

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Jeremy W. D. McGowan¹, Gene L. Bidwell, III¹

¹Department of Neurology, The University of Mississippi Medical Center

Ex vivo whole-organ imaging is a rapid method for determining the relative concentrations of fluorescently labeled compounds within and between tissues or treatment groups. Conversely, quantitative fluorescence histology, while more labor intensive, allows for the quantification of the absolute tissue levels of labeled molecules.

JoVE Core

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

Sarah M. Peterson¹, Xuexiang Wang², Ashley C. Johnson¹, Ian D. Coate¹, Michael R. Garrett^1,3, Sean P. Didion^1,4

¹Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, ²Department of Medicine, Division of Nephrology, Rush University Medical Center, ³Department of Medicine, Division of Nephrology, The University of Mississippi Medical Center, ⁴Department of Neurology, The University of Mississippi Medical Center

Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.