AFM and Microrheology in the Zebrafish Embryo Yolk CellMaria Marsal 1, Ignasi Jorba 2, Elena Rebollo 1, Tomas Luque 2, Daniel Navajas 2, Enrique Martín-Blanco 1
1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
