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Mass General Institute of Neurodegenerative Disease

3 ARTICLES PUBLISHED IN JoVE

Biology

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Jing Men^1,2, Jason Jerwick^2,3, Penghe Wu^1,2, Mingming Chen^3,4, Aneesh Alex^2,3, Yutao Ma⁴, Rudolph E. Tanzi⁵, Airong Li⁵, Chao Zhou^1,2,3

¹Bioengineering Program, Lehigh University, ²Center for Photonics and Nanoelectronics, Lehigh University, ³Department of Electrical and Computer Engineering, Lehigh University, ⁴State Key Laboratory of Software Engineering, Wuhan University, ⁵Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.

Neuroscience

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Shanshan Wang^1,2, Rudolph E. Tanzi¹, Airong Li¹

¹Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital, Mass General Institute of Neurodegenerative Disease, ²Department of Geriatric Neurology, Nanlou Clinical Division, PLA General Hospital

This protocol focuses on quantitative analysis of neuronal dendritic arborization complexity (NDAC) in Drosophila, which can be used for studies of dendritic morphogenesis.

Immunology and Infection

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Fengwei Zhang¹, Yanan Wang², Xuechai Shang¹, Shanshan Wang¹, Rong Xiao¹, Hongjuan Zhou¹, Long Cai¹

¹Central Laboratory, Hang Zhou Red Cross Hospital, ²Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.