Iniciar sesión

Mass General Institute of Neurodegenerative Disease

3 ARTICLES PUBLISHED IN JoVE

image

Biology

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
Jing Men 1,2, Jason Jerwick 2,3, Penghe Wu 1,2, Mingming Chen 3,4, Aneesh Alex 2,3, Yutao Ma 4, Rudolph E. Tanzi 5, Airong Li 5, Chao Zhou 1,2,3
1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.

image

Neuroscience

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
Shanshan Wang 1,2, Rudolph E. Tanzi 1, Airong Li 1
1Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital, Mass General Institute of Neurodegenerative Disease, 2Department of Geriatric Neurology, Nanlou Clinical Division, PLA General Hospital

This protocol focuses on quantitative analysis of neuronal dendritic arborization complexity (NDAC) in Drosophila, which can be used for studies of dendritic morphogenesis.

image

Immunology and Infection

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
Fengwei Zhang 1, Yanan Wang 2, Xuechai Shang 1, Shanshan Wang 1, Rong Xiao 1, Hongjuan Zhou 1, Long Cai 1
1Central Laboratory, Hang Zhou Red Cross Hospital, 2Clinical Laboratory, Yuhang District Maternity and Child Health Care Hospital

This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.

JoVE Logo

Privacidad

Condiciones de uso

Políticas

Investigación

Educación

ACERCA DE JoVE

Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados