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1 ARTICLES PUBLISHED IN JoVE

Medicine

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Suzan Wetzels^*1,2,3, Mitchell Bijnen^*1,2, Erwin Wijnands^2,4, Erik A.L. Biessen^2,4, Casper G. Schalkwijk^1,2, Kristiaan Wouters^1,2

¹Department of Internal Medicine, MUMC, ²CARIM, MUMC, ³Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, ⁴Department of Pathology, MUMC

This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.