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University of Edinburgh, Queens Medical Research Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Iwan R. Evans¹, Jennifer Zanet², Will Wood¹, Brian M. Stramer²

¹Department of Biology and Biochemistry, University of Bath, ²Randall Division of Cell and Molecular Biophysics, King's College London

Drosophila hemocytes disperse over the entirety of the developing embryo. This protocol demonstrates how to mount and image these migrations using embryos with fluorescently labelled hemocytes.

Immunology and Infection

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Helen Weavers^1,2, Anna Franz¹, Will Wood³, Paul Martin^1,4

¹School of Biochemistry, Biomedical Sciences, University of Bristol, ²School of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, ³MRC Centre for Inflammation Research, University of Edinburgh, Queens Medical Research Institute, ⁴School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences, University of Bristol

Here we present a protocol for live-imaging wound repair and the associated inflammatory response at high spatio-temporal resolution in vivo. This method utilizes the pupal stage of Drosophila development to enable long-term imaging and tracking of specific cell populations over time and is compatible with efficient RNAi-mediated gene inactivation.