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Charité University Medicine

1 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

David Bode^1,2, Tim Guthof¹, Burkert M. Pieske^1,2, Frank R. Heinzel^1,2, Felix Hohendanner^1,2

¹Department of Internal Medicine and Cardiology, Charité University Medicine, ²German Center for Cardiovascular Research (DZHK)

Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.