Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection FractionDavid Bode 1,2, Tim Guthof 1, Burkert M. Pieske 1,2, Frank R. Heinzel 1,2, Felix Hohendanner 1,2
1Department of Internal Medicine and Cardiology, Charité University Medicine, 2German Center for Cardiovascular Research (DZHK)
Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.
