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Graduate Center, City University of New York

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Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Irina E. Catrina¹, Livia V. Bayer^1,2, Omar S. Omar^1,2, Diana P. Bratu^1,2

¹Biological Sciences Department Hunter College, City University of New York, ²Program in Molecular, Cellular, and Developmental Biology, Graduate Center, City University of New York

Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.