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UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Camille Bouillier¹, Vincent Rincheval¹, Delphine Sitterlin¹, Sabine Blouquit-Laye¹, Aurore Desquesnes¹, Jean-François Eléouët², Elyanne Gault^1,3, Marie-Anne Rameix-Welti^1,3

¹UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, ²UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, ³Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.