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Maynooth University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Ingo Bauer¹, Angelo Pidroni¹, Özgür Bayram^2,3, Gerald Brosch¹, Stefan Graessle¹

¹Division of Molecular Biology, Biocenter, Medical University of Innsbruck, ²Biology Department, Maynooth University, ³Maynooth University Human Health Research Institute

Class 1 histone deacetylases (HDACs) like RpdA have gained importance as potential targets to treat fungal infections. Here we present a protocol for the specific enrichment of TAP-tagged RpdA combined with an HDAC activity assay that allows in vitro efficacy testing of histone deacetylase inhibitors.

Immunology and Infection

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Alessandro Donada¹, Tamar Tak¹, Giulio Prevedello^1,2,3,4, Idan Milo¹, Ken R. Duffy², Leïla Perié¹

¹Quantitative Immuno-hematology, CNRS UMR168, Institut Curie, ²Hamilton Institute, Maynooth University, ³Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, ⁴Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.