Maynooth University
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Ingo Bauer 1, Angelo Pidroni 1, Özgür Bayram 2,3, Gerald Brosch 1, Stefan Graessle 1
1Division of Molecular Biology, Biocenter, Medical University of Innsbruck, 2Biology Department, Maynooth University, 3Maynooth University Human Health Research Institute
Class 1 histone deacetylases (HDACs) like RpdA have gained importance as potential targets to treat fungal infections. Here we present a protocol for the specific enrichment of TAP-tagged RpdA combined with an HDAC activity assay that allows in vitro efficacy testing of histone deacetylase inhibitors.
Immunology and Infection
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Alessandro Donada 1, Tamar Tak 1, Giulio Prevedello 1,2,3,4, Idan Milo 1, Ken R. Duffy 2, Leïla Perié 1
1Quantitative Immuno-hematology, CNRS UMR168, Institut Curie, 2Hamilton Institute, Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay
Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.
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