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National Research Council of Italy (CNR)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Methods to Test Endocrine Disruption in Drosophila melanogaster

Tiziana Francesca Bovier¹, Daniela Cavaliere¹, Michele Colombo¹, Gianfranco Peluso², Ennio Giordano³, Filomena Anna Digilio^1,2

¹Institute of Biosciences and BioResources, National Research Council of Italy (CNR), ²Institute of Research on Terrestrial Ecosystems (IRET), National Research Council of Italy (CNR), ³Department of Biology, UniNa "Federico II"

Endocrine disruptor chemicals (EDCs) represent a serious problem for organisms and for natural environments. Drosophila melanogaster represents an ideal model to study EDC effects in vivo. Here, we present methods to investigate endocrine disruption in Drosophila, addressing EDC effects on fecundity, fertility, developmental timing, and lifespan of the fly.

Immunology and Infection

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Sonia Simonetti^*1,2, Ambra Natalini^*1,2, Giovanna Peruzzi³, Alfredo Nicosia⁴, Antonella Folgori⁵, Stefania Capone⁵, Angela Santoni^2,6, Francesca Di Rosa¹

¹Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), ²Department of Molecular Medicine, University of Rome “Sapienza”, ³Center for Life Nano Science, Istituto Italiano di Tecnologia, ⁴Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, ⁵Reithera Srl, ⁶IRCCS, Neuromed

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.