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Helsinki University Hospital

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Erika Naakka¹, Katja Tuomainen¹, Henrik Wistrand^*1, Miila Palkama^*1, Ilida Suleymanova¹, Ahmed Al-Samadi¹, Tuula Salo^1,2,3,4

¹Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, ²Cancer and Translational Medicine Research Unit, University of Oulu, ³Medical Research Center, Oulu University Horspital, ⁴Helsinki University Hospital

Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.

Genetics

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Snehadri Sinha^*1,2, Bettina Mannerström^*1,2, Riitta Seppänen-Kaijansinkko^1,2, Sippy Kaur^1,2

¹Department of Oral and Maxillofacial Diseases, University of Helsinki, ²Helsinki University Hospital

Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.