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Helsinki University Hospital

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Erika Naakka 1, Katja Tuomainen 1, Henrik Wistrand *1, Miila Palkama *1, Ilida Suleymanova 1, Ahmed Al-Samadi 1, Tuula Salo 1,2,3,4
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, 2Cancer and Translational Medicine Research Unit, University of Oulu, 3Medical Research Center, Oulu University Horspital, 4Helsinki University Hospital

Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.

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Genetics

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
Snehadri Sinha *1,2, Bettina Mannerström *1,2, Riitta Seppänen-Kaijansinkko 1,2, Sippy Kaur 1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital

Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.

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