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Nanosystems Initiative Munich

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Günther Pardatscher^*1, Matthaeus Schwarz-Schilling^*1, Sandra Sagredo¹, Friedrich C. Simmel¹

¹Physics Department, Technical University of Munich

We describe a simple lithographic procedure for the immobilization of gene-length DNA molecules on a surface, which can be used to perform cell-free gene expression experiments on biochips.

Bioengineering

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Kilian Vogele¹, Thomas Frank¹, Lukas Gasser¹, Marisa A. Goetzfried¹, Mathias W. Hackl², Stephan A. Sieber², Friedrich C. Simmel^1,3, Tobias Pirzer¹

¹Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, ²Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, ³Nanosystems Initiative Munich

Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.