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University of California Irvine Health

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Saahir Khan¹, Aarti Jain², Omid Taghavian², Rie Nakajima², Algis Jasinskas², Medalyn Supnet², Jiin Felgner², Jenny Davies², Rafael Ramiro de Assis², Sharon Jan², Joshua Obiero², Erwin Strahsburger², Egest J. Pone², Li Liang², D. Huw Davies², Philip L. Felgner²

¹Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, ²Vaccine Research and Development Center, Department of Physiology, University of California Irvine

We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.