Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus SubtypesSaahir Khan 1, Aarti Jain 2, Omid Taghavian 2, Rie Nakajima 2, Algis Jasinskas 2, Medalyn Supnet 2, Jiin Felgner 2, Jenny Davies 2, Rafael Ramiro de Assis 2, Sharon Jan 2, Joshua Obiero 2, Erwin Strahsburger 2, Egest J. Pone 2, Li Liang 2, D. Huw Davies 2, Philip L. Felgner 2
1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.
