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University of Ottawa Heart Institute

3 ARTICLES PUBLISHED IN JoVE

Medicine

Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior

Matthew M. Piaseczny^*1,2, Graciella M. Pio^*1,2, Jenny E. Chu^1,2, Ying Xia¹, Kim Nguyen^1,3, David Goodale¹, Alison Allan^1,2,4,5

¹London Regional Cancer Program, London Health Sciences Centre, ²Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, Western University, ³Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, ⁴Department of Oncology, Schulich School of Medicine & Dentistry, Western University, ⁵Lawson Health Research Institute

This manuscript describes an ex vivo model system comprised of organ-conditioned media derived from the lymph node, bone, lung, and brain of mice. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.

Biology

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Ri Youn Kim^*1,2, Ju Hee Lee^*3,4, Yena Oh^1,2, Hoon-Ki Sung^3,4,5, Kyoung-Han Kim^1,2

¹University of Ottawa Heart Institute, ²Department of Cellular and Molecular Medicine, University of Ottawa, ³Translational Medicine Program, The Hospital for Sick Children, ⁴Department of Laboratory Medicine and Pathobiology, University of Toronto, ⁵Banting and Best Diabetes Centre, University of Toronto

The current article describes a detailed protocol for isocaloric 2:1 intermittent fasting to protect and treat against obesity and impaired glucose metabolism in wild-type and ob/ob mice.

Biology

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Aizhu Lu^1,2, Jerry Wang^1,2, Ying Xia^1,2, Ruonan Gu¹, Kyoung-Han Kim^1,2, Erin E. Mulvihill^1,3, Darryl R. Davis^1,2, Rob S. Beanlands¹, Wenbin Liang^1,2

¹University of Ottawa Heart Institute, ²Department of Cellular and Molecular Medicine, University of Ottawa, ³Department of Biochemistry, Microbiology and Immunology, University of Ottawa

The present protocol describes methods for transgene expression in rat and mouse hearts by direct intramyocardial injection of the virus under echocardiography guidance. Methods for the assessment of the susceptibility of hearts to ventricular arrhythmias by the programmed electrical stimulation of isolated, Langendorff-perfused hearts are also explained here.