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Japan Society for the Promotion of Science

2 ARTICLES PUBLISHED IN JoVE

Behavior

A Task for Assessing the Impact of a Partner on the Speed and Accuracy of Motor Performance in Rats

¹Graduate School of Psychology, Doshisha University, ²Japan Society for the Promotion of Science, ³Faculty of Psychology, Doshisha University

A procedure to measure the speed and accuracy of rats’ motor performance in a social condition is described. The protocol enables us to investigate the effect of the mere presence of others on speed and accuracy of motor performance in one experiment.

Neuroscience

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Kenta Yamauchi^1,2, Shinichiro Okamoto^1,2,3, Megumu Takahashi^1,2,4,5, Masato Koike^2,3, Takahiro Furuta⁶, Hiroyuki Hioki^1,2,7

¹Department of Neuroanatomy, Juntendo University Graduate School of Medicine, ²Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, ³Advanced Research Institute for Health Sciences, Juntendo University, ⁴Department of Neuroscience, Graduate School of Medicine, Kyoto University, ⁵Japan Society for the Promotion of Science, ⁶Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, ⁷Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic levels.