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Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM)

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Javier Traba^*1, Pietro Miozzo^*2, Billur Akkaya³, Susan K. Pierce², Munir Akkaya²

¹Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, ²Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ³Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health

The study of metabolism is becoming increasingly relevant to immunological research. Here, we present an optimized method for measuring glycolysis and mitochondrial respiration in mouse splenocytes, and T and B lymphocytes.

Immunology and Infection

Analysis of Human Natural Killer Cell Metabolism

Javier Traba^1,2, Thomas A. Waldmann³, Olga M. Anton³

¹Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, ²Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), ³Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.