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The City College of New York and The Graduate Center of The City University of New York

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Emily Sible¹, Simin Zheng², Jee Eun Choi¹, Bao Q. Vuong¹

¹Department of Biology, The City College of New York and The Graduate Center of The City University of New York, ²Department of Microbiology, Icahn School of Medicine at Mount Sinai

Presented here is an assay to quantify somatic hypermutation within the immunoglobulin heavy chain gene locus using germinal center B cells from mouse Peyer’s patches.