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Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Mark D Tully^*1, Nicolas Tarbouriech², Robert P Rambo³, Stephanie Hutin^*4

¹European Synchrotron Radiation Facility Structural Biology Group, Structural Biology Group, ²Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, ³Diamond Light Source, ⁴Laboratoire de Physiologie Cellulaire and Végétale, Université Grenoble Alpes/CNRS/CEA/INRA/BIG

SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.