University of Bristol, Bristol, UK

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Efficient and Scalable Generation of Human Ventral Midbrain Astrocytes from Human-Induced Pluripotent Stem Cells

Lucy A. Crompton¹, Sarah F. McComish², Petros Stathakos¹, Oscar Cordero-Llana³, Jon D. Lane^*1, Maeve A. Caldwell^*2

¹Cell Biology Laboratories, School of Biochemistry, University of Bristol, Bristol, UK, ²Department of Physiology and Trinity College Institute of Neuroscience, Trinity College, Dublin, Ireland, ³Regenerative Medicine Laboratory, School of Clinical Sciences, University of Bristol, Bristol, UK

Here, we present a method for reproducible generation of ventral midbrain patterned astrocytes from hiPSCs and protocols for their characterization to assess phenotype and function.