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University of Bristol, Bristol, UK

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Efficient and Scalable Generation of Human Ventral Midbrain Astrocytes from Human-Induced Pluripotent Stem Cells
Lucy A. Crompton 1, Sarah F. McComish 2, Petros Stathakos 1, Oscar Cordero-Llana 3, Jon D. Lane *1, Maeve A. Caldwell *2
1Cell Biology Laboratories, School of Biochemistry, University of Bristol, Bristol, UK, 2Department of Physiology and Trinity College Institute of Neuroscience, Trinity College, Dublin, Ireland, 3Regenerative Medicine Laboratory, School of Clinical Sciences, University of Bristol, Bristol, UK

Here, we present a method for reproducible generation of ventral midbrain patterned astrocytes from hiPSCs and protocols for their characterization to assess phenotype and function.

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