Imperial College London, South Kensington Campus

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Natàlia de Martín Garrido¹, Kailash Ramlaul¹, Christopher H. S. Aylett¹

¹Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Charles P. Brown^1,2, Alan Armstrong¹, David J. Mann²

¹Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, ²Department of Life Sciences, Imperial College London, South Kensington Campus

The quantitative irreversible tethering (qIT) assay is a fluorescence-based method of identifying covalent fragments that selectively engage with a target protein. Here, a detailed protocol is outlined to describe the qIT assay, with example results included.