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Imperial College London, South Kensington Campus

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Natàlia de Martín Garrido¹, Kailash Ramlaul¹, Christopher H. S. Aylett¹

¹Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.