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Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges

1 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Janet Y. Sheung¹, Megan Otsuka², Gabriella Seifert³, Athena Lin⁴, Wallace F. Marshall⁴

¹W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, ²W. M. Keck Science Department, Pitzer College, ³W. M. Keck Science Department, Scripps College, ⁴Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.