Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell TrackingJanet Y. Sheung 1, Megan Otsuka 2, Gabriella Seifert 3, Athena Lin 4, Wallace F. Marshall 4
1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco
We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.
