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Champalimaud Centre for the Unknown, Champalimaud Foundation

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Mayra Martinez-Lopez^1,2, Vanda Póvoa¹, Rita Fior¹

¹Champalimaud Centre for the Unknown, Champalimaud Foundation, ²Instituto Gulbenkian de Ciência

Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.