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The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Huiqin Tang^*1, Hui Xie^*1, Zhun Wang¹, Shuanghe Peng², Wenli Ni¹, Linpei Guo³

¹Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, ²Department of Pathology, The Second Hospital of Tianjin Medical University, ³Department of Urology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.