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Centro Nacional de Investigaciones Cardiovasculares (CNIC)

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats

Marta Casquero-Veiga¹, Nicolás Lamanna-Rama^2,3, Diego Romero-Miguel^2,3, Manuel Desco^1,2,3,4, María Luisa Soto-Montenegro^2,4,5

¹Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Laboratorio de Imagen Médica, Instituto de Investigación Sanitaria Gregorio Marañón, ³Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, ⁴CIBER de Salud Mental (CIBERSAM), ⁵High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut), Universidad Rey Juan Carlos

We describe a preclinical experimental method to evaluate metabolic neuromodulation induced by acute deep brain stimulation with in vivo FDG-PET. This manuscript includes all experimental steps, from stereotaxic surgery to the application of the stimulation treatment and the acquisition, processing, and analysis of PET images.

Developmental Biology

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Tamara Gonzalez-Costa^1,2, Jose Luis de la Pompa^1,2, Joaquim Grego-Bessa^1,2

¹Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Ciber de Enfermedades Cardiovasculares

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

Developmental Biology

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Miquel Sendra¹, Jorge Mañes¹, Jorge N. Domínguez^2,3, Miguel Torres¹

¹Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, ³Fundación Medina, Granada

We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.