Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway OrganoidsCun Li 1,2, Man Chun Chiu 1,2, Yifei Yu 1,2, Xiaojuan Liu 1,2, Ding Xiao 1,2, Jingjing Huang 1,2, Zhixin Wan 1,2, Jie Zhou 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong
The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.
